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G. Madhava Prathap* and Amreen Nishat

Ultra performance liquid chromatography systems take advantage of technological pace in particle chemistry performance, system optimization, detector design and data processing. When taken together, these achievements have created an improvement in chromatographic performance. This new category of analytical separation science retains the practicality and principles of HPLC while increasing the overall interrelated attributes of speed, sensitivity and resolution. Today’s pharmaceutical industries are looking for new ways to cut cost and shorten time for development of drugs while at the same time improving the quality of their products and analytical laboratories are not exception in this trend. Speed allows a greater number of analyses to be performed in a shorter amount of time thereby increasing sample throughput and lab productivity. These are the benefits of faster analysis and hence the ultra performance liquid chromatography. A typical assay was transferred and optimized for UPLC system to achieve both higher sample analysis throughput and better assay sensitivity. Analysis of operation cost and sample throughput found UPLC cost advantageous over HPLC

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