Background: Sickle cell disease is an inherited disorder caused by the single point mutation due to the replacement of valine for glutamic acid at the sixth codon of β-chain of globins (β6Glu → Val). The sickle cell disease condition with hepatic dysfunction shows the abnormal variations in liver function tests including serum aminotransferases enzymes like SGOT and SGPT level were changed and to discuss their significance in sickle cell disease.
Materials and methods: This study was being conducted in the Department of Biochemistry, People’s College of Medical Sciences and Research Centre (PCMS and RC), and Centre for Scientific Research and Development Department (CSRD), People’s University, Bhopal. The study protocol and ethical clearance had been approved by Institutional Ethical Committee (Reference number: PCMS/OD/2016/2551). The sample size was estimated by the expert statistician, which were 111 for case sample and controls. These were employed in the research study after the application of the inclusion and exclusion criteria.
The liver function enzymes SGOT and SGPT were estimated by Reitman and Frankel’s method and were diagnosed by using the Trans Asia diagnostic kits manufactured by TRANSASIA BIO- MEDICALS LTD., B-11, and OIDC. RINGANWADA, DAMAN-396210 (India) in collaboration with ERBA diagnostics Mannheim HmbH Mallaustr. 69-73, D-68291, Mannheim/Germany
Results: In the present study, the mean standard deviations of cases vs. controls were showed significant difference and it was calculated by using the SPSS latest software version-24.
The Mean ± SD cases vs. controls of SGOT and SGPT were found in 69.98 ± 69.31; 25.17 ± 5.25 and 65.28 ± 60.07; 22.72 ± 5.47 respectively. The calculated p?0.000 was found to be statistically highly significant. The Pearson’s Correlation showed positive correlation in between SGOT and SGPT enzymes.
Conclusion: The transaminases SGOT-SGPT enzymes were significantly increased in sickle cell disease so it could be another clinical bio-marker for the diagnosis of sickle cell disease.
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