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Abstract

LIQUID CHROMATOGRAPHY - MASS SPECTROMETRY AND ITS APPLICATIONS

Renuka Jajikore*, G. Ushasree, A. Ajitha, V. Umamaheswararao

Liquid chromatography is a fundamental separation technique in the life sciences and related fields of chemistry. An LC-MS is an HPLC system with a mass spec detector. Liquid chromatography-mass spectrometry is now a routine technique with the development of electrospray ionisation providing a simple and robust interface. It can be applied to a wide range of biological molecules and the use of tandem MS and stable isotope internal standards allows highly sensitive and accurate assays to be developed although some method optimisation is required to minimise ion suppression effects.


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