P.S.R.CH.N.P. Varma D, A. Lakshmana Rao* and S.C. Dinda
A simple, precise and rapid HPLC method has been developed for the simultaneous determination of Perindopril and Indapamide in pharmaceutical dosage form. The method was carried out using Hypersil BDS C18 column (250 mm x 4.6 mm, 5μm) and mobile phase comprised of phosphate buffer pH 3.5±0.05 and methanol in the ratio of 65:35 v/v and degassed under ultrasonication. The flow rate was 1.0 mL/min and the effluent was monitored at 215 nm. The retention times of Perindopril and Indapamide were 3.53 min and 4.09 min respectively. The method was validated in terms of linearity, precision, accuracy, specificity, limit of detection, limit of quantitation and by performing recovery study. Linearity was in the range of 160 to 480 μg/mL for Perindopril and 50 to 150 μg/mL for Indapamide respectively. The percentage recoveries of both the drugs were ranging from 97.8 to 101.7 for Perindopril and 98.7 to 101.8 for Indapamide respectively from the tablet formulation. The proposed method is suitable for the routine quality control analysis of simultaneous determination of Perindopril and Indapamide in bulk and pharmaceutical dosage form.
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