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Abstract

UPLC METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF CEFPIROME SULPHATE IN PHARMACEUTICAL DOSAGE FORM

Satish Pavuluri*, Sridhar Siddiraju

A simple, rapid, and precise UPLC method is developed for the quantitative determination of Cefpirome sulphate in pharmaceutical dosage form. The chromatographic separation of Cefpirome sulphate was achieved with an phenomnex c18 (2.5 X 100 mm, 3.0 μm) analytical column using 0.01M phosphate buffer and acetonitrile taken in 50:50%v/v and the response was detected at 265 nm by using PDA detector. The retention time was found to be 0.652 min. The described method shows excellent linearity over a range of 7.5-75 μg/mL. The correlation coefficient for Cefpirome sulphate was found to be 0.999. The relative standard deviation for six measurements in two sets of Cefpirome sulphate in injection is always less than 2%. The proposed method was found to be suitable and accurate for quantitative determination of Cefpirome sulphate in pharmaceutical preparations.


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Citations : 1101

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