Total phenol, total flavonoid content and antioxidant activity of methanol extract of Lygodium palmatum (Bernh.) Sw. leaves.

Tanvir Ahmad Chowdhury, Mohammad Shah Hafez Kabir*, Syed Md. Abdul Kader, Mahmudul Hasan, Md. Ariful Islam, Md. Rakibul Hasan, Md. Nazmul Huda, Shefatun Nur, Joy Chakraborty, Abul Hasanat, Md. Mominur Rahman

Lygodium palmatum (Bernh.) Sw. leaves are deliberated as worthy traditional medicine. To give a scientific basis for traditional usage of this medicinal plant, the leaf extract were appreciated for its antioxidant activity. In this study, in vitro antioxidant activities of the leaf extract of L. palmatum were determined by total antioxidant, DPPH, Reducing power capacity, total phenolic content, total flavonoid content. The extract showed a dose dependent radical scavenging effect in DPPH assay. IC50 for free radicals achieved by the extract is 46.94 μg/ml. The extract showed significant reducing power activity as compared to ascorbic acid and proportionally increased with the increasing concentration of the extract. Increase in absorbance of the reaction mixture indicates the increase in the reducing power of the extract. Phenol content was 96.67± 0.82 mg gallic acid/g and flavonoid content was 27.30 ± 0.30 mg quercetin/g. Our current results emerged that L. palmatum act as an antioxidant agent due to its free radical scavenging and cytoprotective activity. So, the plant may be further pursued to find out for its pharmacological active natural products.