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Abstract

SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF EPALRESTAT AND METHYLCOBALAMIN IN PHARMACEUTICAL DOSAGE FORM

Madhuri A. Hinge*, Niraj K.Patel, Rajvi J. Mahida

A simple, accurate, and reproducible UV-spectrophotometric method has been developed for simultaneous estimation of Epalrestat and Methylcobalamin in tablet dosage form. In Absorbance ratio method ,two wavelengths 238 nm and 257.80 nm were used over the concentration range of 50 - 250 μg/ml and 0.5 - 2.5 μg/ml for Epalrestat and Methylcobalamine respectively. Correlation coefficient found to be 0.9995.Accuracy for both the drugs were in the range of 98-101 %. The method was validated as per the International Conference on Harmonization (ICH) guidelines.


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