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Simultaneous Estimation of Sofosbuvir and Velpatasvir in Bulk and Dosage Form by UV Spectrophotometry

Raviraj Nagnath Jalkote*

Simple, sensitive, specific and economic spectrophotometric method was developed and validated for simultaneous quantitation of sofosbuvir and velpatasvir in tablet dosage form. New method based on the simultaneous estimation of drugs in a binary mixture without previous separation was developed. In simultaneous equation method, sofosbuvir and velpatasvir were quantified using their absorptivity values of at selected wavelengths. The drug obeyed the Beer’s law and shows good correlation near to r2=0.999 for sofosbuvir 2r and for velpatasvir r2=0.998. Beer’s law was obeyed in concentration range of 5-25 μg/ml for sofosbuvir and 10-50 μg/ml for velpatasvir. The method has been validated for linearity, accuracy and precision. The recovery was 100.6% for sofosbuvir and 98.9% for velpatasvir. The developed method was found to be accurate, simple, precise, economical, and selective for simultaneous estimation of sofosbuvir and velpatasvir tablet dosage form.

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