PRECLINICAL LIPID PROFILE STUDIES OF A CLASSICAL AYURVEDIC PREPARATION MAKARADHWAJ RAS AFTER CHRONIC ADMINISTRATION TO MALE SPRAGUE-DAWLEY RATS

Uday Krishna Mondal1, Md. Afaz Uddin1, Sagor Chandra Roy1, Tanmoy Sana1, Md. Mamun Sikder1, Marjana Khalil2, Md. Nuruzzaman Neon1and M. S. K. Choudhuri1*

Makaradhwaj Ras (MRS) is an Ayurvedic preparation used in traditional medicine as a cure for debility in chronic fatigue syndrome in the rural population. The present study is conducted to evaluate the effect of conventionally prepared MRS on different lipid profile parameters in experimental animals. Acute toxicity tests were conducted to determine the LD50 of the drug. To find out the effect of chronic administration of MRS on serum lipid profile it was administered chronically to the male Sprague-Dawley rats at a dose of 40 mg/kg for 28 days. The drug (MRS) did not affect triglyceride (TG), total cholesterol (TC), LDL-C, VLDL-C, HDL-C and Non HDL-C level significantly; thus leading to an insignificant change in atherogenic indices like Cardiac Risk Ratio (TC/HDL-C), Atherogenic Coefficient [(TC - HDL-C)/HDL-C)], Castelli’s Risk Index-II (CRI-II) (HDL-C/LDL-C) and Atherogenic Index of Plasma (AIP) (log (TG/HDL-C)). This experimental data will help the clinician for the logical use of MRS in different disease conditions with findings like no significant change in the lipid profile parameters.