Phytochemical Screening and Pre-Clinical Evaluation of Analgesic, AntiInflammatory and Antipyretic Potentials of the Ethanolic Extract of Wedelia Trilobata

Al Foyjul Islam, Runa Masuma, Anindya Barua, Masum Ahmed, Ataur Rahman, Asrafuzzaman Sobuj, Ersha

Plants have been used as a source of natural products for various therapeutic purposes for a long time. Focus on plant research has increased all over the world due to easy accessibility and less adverse effect with much more contribution in various traditional systems of medicines. Wedelia trilobata (WT) has been used in folk medicine to alleviate several diseases such as cough, colds, flu, fevers and inflammations. The present study was investigated to evaluate the analgesic, anti-inflammatory and antipyretic effect of Wedelia trilobata. Swiss albino mice of either sex weighing 25-30 gm were used in this study. Analgesic activity was evaluated by using hot plate test, acetic acid induced writhing test and formalin induced paw licking test. Anti-inflammatory effect was assessed using cotton pellet and xylene test and carrageenan induced paw edema test. The antipyretic effect was investigated using yeast induced pyrexia test. The extract was used at 1000 and 2000 mg.kg body weight. The WT ethanolic extract showed significant effect against pain in acetic acid (p<0.01) and formalin test (p<0.001) and insignificantly in hot plate test. Inflammation was reduced by WT extract in cotton pellet test (p<0.001), xylene test (p<0.01) and significantly reduced the paw edema (p<0.001) in carrageenan induced paw edema test. Treatment with WT at both the doses reduced pyrexia very highly significantly (p<0.001) in yeast induced antipyretic test. Results from the studies suggested that the WT extract showed potent analgesic, anti-inflammatory and antipyretic effects in various animal model.