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Abstract

IN-VITRO ANTIBACTERIAL, ANTIFUNGAL AND ANTHELMINTHIC ACTIVITY OF THE BARK OF MADHUCA INDICA

K. Pavan Kumar, Balisa Mosisa Ejeta, Biniam Paulos Bura, Madhusudhana Reddy Induri

The research work was carried on the evaluation of antibacterial, antifungal and anthelminthic activities of the methanolic, petroleum ether and aqueous extracts of Madhuca indica bark belonging to family Sapotaceae. Disc diffusion technique was used for in-vitro antibacterial and antifungal activities using ciprofloxacin (5µg/disc) and clotrimazole (10µg/disc), zone of inhibition was observed against two gram positive bacteria (Staphylococcus aureus, Bacillus subtilis), two gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa), two fungal species (Aspergillus Niger, Candida albicans) at concentration of 50, 100, 150, 200µg/mL). Anthelminthic activity was investigated in pheretima posthuma (Indian Earth Worm) at concentration 10, 20, 50mg/mL against standard drug piperazine citrate (10mg/mL). For antibacterial and antifungal activity of all the extracts has shown moderate to good activity against standard drugs. Among all the extracts aqueous extract and methanolic extract have shown good activity when compared to petroleum ether extract which showed moderate activity against standard drugs. For anthelminthic activity of petroleum ether extract has shown good activity against the other extracts.


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