GET THE APP

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

IN VITRO ANTIBACTERIAL ACTIVITY OF ROSMARINUS OFFICINALIS METHANOLIC AND AQUEOUS EXTRACTS

Rachad Alnamer*, Katim Alaoui, Latifa Doudach, El Houcine Bouidida, Fatiha Chibani, Mosa√Ę??d AL-Sobarry, Abdelaziz Benjouad and Yahia Cherrah

Rosemary (Rosmarinus officinalis L., family Lamiaceae) had been reported in traditional medicine, to exhibit antimicrobial properties. Therefore, this study is aimed at determining the antibacterial activity of R. officinalis leaves against pathogenic microorganisms by determination the minimal inhibitory concentration and to serve as criteria to recommend the ethno pharmacological uses of the plant. Plant leaves were dried, powdered and extracted by cold maceration with methanol for 48h. The extracts were screened against 24h broth culture of bacteria seeded in Muller Hinton Agar at concentration 200, 100, 50, 25, 12.5, 6.25, 3.125 and 1.56mg/ml in sterile distilled water and incubated at 37°C, for 18h and measuring the inhibition zone diameter (IZD). Minimum inhibitory concentrations (MICs) against three Gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus and Bacillus subtilis), three Gram-negative bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli) were determined for the methanolic and aqueous extracts of Rosmarinus officinalis. The methanolic extract showed pronounced antibacterial than the aqueous extract against all of the tested microorganisms. Methanolic extract inhibited with minimal inhibitory concentration of 1.56, 1.56, 3.13, 1.56, 3.13 and 3.13mg/ml against Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, respectively, while the aqueous extract inhibited with minimal inhibitory concentration of 6.25 mg/ml against all tested bacterial strains both Gram-positive and Gram-negative bacteria. The methanolic and aqueous extracts of R. officinalis demonstrated activities against certain bacteria confirming the use of the plant in ethno pharmacology.


PDF

Manuscript Submission

Submit your manuscript at  https://www.scholarscentral.org/submissions/international-pharmacy.html


Google scholar citation report
Citations : 938

International Journal of Pharmacy received 938 citations as per google scholar report

International Journal of Pharmacy peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • HINARI
  • Google Scholar
  • The Global Impact Factor (GIF)
  • Polish Scholarly Bibliography (PBN)
  • Cosmos IF
  • Open Academic Journals Index (OAJI)
  • Directory of Research Journal Indexing (DRJI)
  • EBSCO A-Z
  • International committee of medical journals editors (ICMJE)
  • Scientific Indexing Services (SIS)
  • Scientific Journal Impact Factor (SJIF)
  • Euro Pub
  • Eurasian Scientific Journal Index
  • Root indexing
  • International Institute of Organized Research
  • InfoBase Index
  • International Innovative Journal Impact Factor