Effect of Ashwagandharista on Different Hepatic Enzymes at Three Different Dose Level Utilizing Male and Female Rat Model

Tasmina Rahman1, Md. Rakib Hasan1, Md. Ishtiaq Ahmed Chowdhury1, Md. Mahmudur Rahman Mamun1 and M. S. K. Choudhuri1*

Ashwagandharista (ASG) is a classical Ayurvedic preparation which is used as an anabolic. The key objective of this study was to analyze the effect of ASG on different enzyme profile i.e; Aspartate Aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline Phosphatase (ALP) and Lactate dehydrogenase (LDH). A total of 40 males and 40 females were randomly assigned to the four groups, namely group I (Control: water), group II (0.625 ml/kg BW of ASG), group III (5.0 ml/kg BW of ASG), and group IV (40.0 ml/kg BW of ASG) consisting of 10 males and 10 females in each group. To detect the outcome of ASG on different enzyme profile, it was administered chronically to both male and female Sprague-Dawley rats for 51 days. The results showed significant decrease of serum AST level in ASG treated male rat groups (p < 0.05) than control counterpart. In females, serum AST level in mid dose (group III) was significantly higher as compared to control (p < 0.05). The females from mid dose group showed significant increase in serum ALT level (p < 0.05) whereas other groups from male and female showed no significant changes. For the enzyme Lactate dehydrogenase, only the male rat high dose showed a significant decrease (p < 0.05) than the corresponding control group. No statistically significant change was noted in Alkaline Phosphatase level for both the male and female rats at three different doses.