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Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF SOME DRUGS IN PHARMACEUTICAL FORMULATION

Mahesh H. Kolhe*; Ujwala K. Waghmare; Ramesh D. Bhusal ; Pranit P. Hajare ; Swapnil R Chavan

A simple, accurate and reproducible RP-HPLC method has been developed for simultaneous estimation of Aspirin and Ticlopidine hydrochloride in tablet dosage form. The RP-HPLC analysis is carried out using Acetonitrile: Ammonium acetate buffer (0.05 M) in the ratio of (68: 32 % v/v) as the mobile phase and MOS Thermosil C8 column (250 mm × 4.6 mm i.d.), flow rate 1.0 mL/min, with detection wavelength of 240 nm. Linearity was obtained in the concentration range of 10-50 μg/mL and 20-100 μg/mL for Aspirin and Ticlopidine hydrochloride respectively. The RP-HPLC methods was developed and statistically validated as per ICH guidelines.


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