J. Raghuram*, N. Appalaraju, V. Kiran Kumar
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Azithromycin in tablet dosage form.Xterra RP 18 (250x4.6mm, 5μ particle size), with mobile phase consisting of ortho-phosphori acid: methanol 70:30 V/V was used. The flow rate 1.0 ml/min and the effluents were monitored at 205 nm. The retention time& Recovery time was 12 minutes. The detector response was linear in the concentration of 25–275 μg/ mL. The respective linear regression equation being Y= 1228.302 x+5230.5524. The limit of detection and limit of quantification was 12.5mcg and 37.5mcg/ml respectively. The percentage assay of Azithromycin was 98.0%. The method was validated by determining its accuracy, precision and system suitability.The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Azithromycin in bulk drug and in its pharmaceutical dosage form.
Submit your manuscript at https://www.scholarscentral.org/submissions/international-pharmacy.html