BRINE SHRIMP LETHALITY BIOASSAY, THROMBOLYTIC AND ANTIBACTERIAL ACTIVITIES OF METHANOL EXTRACT OF BOEHMERIA PLATYPHYLLA D DON LEAVES

Md. Saif Uddin, Mohammad Shah Hafez Kabir, Syed Md. Abdul Kader*, Mahmudul Hasan, Md. Abu Aiube Ansary, Md. Mosarraf Hossen, Atiqur Rahman, Mohammad Abdul Awal, Mahmud Mostofa Hridoy, Abul Hasanat, Shaikh Bokhtear Uddin, Md. Masudur Rahman

Extract from the leaves of Boehmeria platyphylla D Don were screened for their brine shrimp lethality bioassay, thrombolytic and antimicrobial activities. The cytotoxicity was surveyed with the brine shrimp lethality bioassay and thrombolytic impact with human blood. The brine shrimp lethality bioassay was utilized to assess cytotoxicity (LC50 = 75.26 μg/ml) contrasted with Vincristine sulfate (LC₅₀ = 0.74 μg/ml). It was also assessed as thrombolytic activity when contrasted with streptokinase. It has significant thrombolytic movement (73.17±2.08%) contrasted with standard streptokinase (81.32±1.46%). The extract indicated zone of inhibition against Gram positive bacteria (Bacillus subtilis and Bacillus cereus) and Gram negative bacteria (Salmonella typhi, Salmonella paratyphi, Escherichia coli, Pseudomonas aeruginosa) at 1000 μg/disc. Gram negative bacteria Staphylococcus aureus demonstrated no action against B. platyphylla leaves extract at both doses. Relative percentage inhibition of the extract against each bacterium also calculated. These results indicated that B. platyphylla have favorable thrombolytic, cytotoxic and antibacterial effects and capacities of B. platyphylla extract to be processed for pharmaceutical use.