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Abstract

BIOASSAY OF BRINE SHRIMP LETHALITY, CARDIOPROTECTIVE AND ANTI-INFLAMMATORY ACTIVITIES OF METHANOLIC LEAF EXTRACT OF FICUS BENJAMINA

Md. Irfan Amin Chowdury*, Mohammad Nazmul Alam, Dewan Shakibuzzaman, Galib Ahsan1, Rajesh Barua, Md. Faruky Azam Chowdhury, Muhammad Moin Uddin Mazumdar, Md. Rabiul Islam

Brine shrimp lethality bioassay, clot lysis and hypotonicity induced membrane lysismethod was used to evaluate the cytotoxic,cardioprotective and anti-inflammatory activity of methanolicextract of Ficus benjamnia. The extract showed moderate cytotoxic activity. LC50 value of the extract was 232.71μg/ml, which was compared with vincristine sulphate (12.59μg/ml). Methanolic leaf extract of Ficus benjamina was treated with human blood to evaluate Cardioprotective effect. It showed promising Cardioprotective activity which was about (34.95± 1.98)% compared to streptokinase which was used as standard (63.54 ± 2.61)%. In the case of anti-inflammatory study, methanolic leaf extract showed (20.79±1.07)% and (58.06±1.03)%of membrane stabilization activity at 31.25μg/ml and 1000 μg/ml concentration respectively.


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