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Antithrombotic, cytotoxic and antibacterial activities of methanol extract of Antidesma ghaesembilla Gaertn leaves.

Syed Md. Abdul Kader*, Mohammad Shah Hafez Kabir, Mahmudul Hasan, Md. Saif Uddin, Md. Abu Aiube Ansary, Muhammad Abdulla Al Noman, Fahima Zaheed, Md. Rabiul Hossain, Mohammad Zia Habib, Md. Ismail Hossain, Abul Hasanat, Md. Rafikul Islam

Extract from the leaves of Antidesma ghaesembilla were screened for their antithrombotic, cytotoxic and antimicrobial exercises. The cytotoxicity was surveyed with the brine shrimp lethality bioassay and antithrombotic impact with human blood. The brine shrimp lethality bioassay was utilized to assess cytotoxicity (LC50 = 432.13 μg/ml) contrasted with Vincristine sulfate (LC50 = 0.74 μg/ml). It was also assessed as antithrombotic activity when contrasted with streptokinase. It has significant antithrombotic movement (63.45±2.08%) contrasted with standard streptokinase (81.32±1.46%). The extract indicated zone of inhibition against Gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and Gram negative bacteria (Salmonella typhi, Salmonella paratyphi, Escherichia coli, Pseudomonas aeruginosa) 1000 μg/disc. Gram negative bacteria Bacillus cereus demonstrated no action against at both doses. A. ghaesembilla leaves extract and relative percentage inhibition of the extract also calculated. These results indicate that A. ghaesembilla have favorable Antithrombotic, cytotoxic and antibacterial effects of A. ghaesembilla extract to be processed for pharmaceutical use.

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