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Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF LORNOXICAM USING RP-HPLC IN BULK DRUG AND FORMULATION

M. Akiful Haque*, S. Hasan Amrohi, A. Manikanta Kumar, Dibyalochan Mohanty and Prakash V Diwan

A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the quantitative determination of Lornoxicam in bulk drug and formulation. The developed method consists of mobile phase, ammonium dihydrogen phosphate Buffer: acetonitrile (50:50) with isocratic programming, Hypersil BDS C18, 250×4.6mm, 5μm column as stationary phase with a flow rate of 1.0 mL/min. Proposed method was found to be linear in the concentration range of 5 – 30 μg/mL, the correlation coefficient was found to be 0.999. System suitability parameters were studied by injecting the standard solution five times and results were well under the acceptance criteria, the proposed method is found to be sensitive, rapid, reproducible, and accurate.


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Citations : 938

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