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Abstract

A VALIDATED HPLC METHOD FOR THE DETERMINATION OF ANAGRELIDE IN PHARMACEUTICAL PREPARATIONS

S. Ramanjaneyulu, A. Durga Vyshnavi and M. K. Chaitanya Prasad

A reversed-phase high-performance liquid chromatographic method is developed for the determination of anagrelide in pharmaceutical preparations. Anagrelide was analysed on a reversed-phase column (XTerra symmetry C18, 150×4.6 mm, 5μm) with a mobile phase containing acetonitrile and water (pH 3.0, pH adjusted with ortho phosphoric acid) in a ratio of 40:60 v/v at a flow-rate of 1.2 mL/min and PDA detection was performed at 250 nm. The retention time anagrelide was 2.349 min. The linearity range was found to be 5-30 μg/mL. The method was validated and it was concluded that the developed method was accurate, sensitive, precise, rugged and useful for the quality control of anagrelide in pharmaceutical preparations.


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Citations : 938

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