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Abstract

A RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF DIHYDRALAZINE IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Saroj Kumar Raul *, B.V.V Ravi kumar, Ajaya Kumar Pattnaik and Nagireddy Neelakanta Rao

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Dihydralazine in pharmaceutical dosage form. Isocratic elution at a flow rate of 0.8 mL min -1 was employed on a Hypersil C18 column at 40oC. The mobile phase consisted of phosphate buffer: acetonitrile 98:2 (v/v) and the detection wavelength was at 305 nm. Linearity was observed in concentration range of 25-150 μg/mL. The retention time for Dihydralazine was 2.75 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Dihydralazine in pharmaceutical dosage forms.


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