Development and validation of RP-HPLC method for the simultaneous estimation of naproxen sodium and esomeprazole magnesium in pharmaceutical tablet dosage form
Srinivas Ampati, SunithaLagishetti, Agaiah Goud Bairi
An isocratic RP-HPLC method was developed and validated for the Simultaneous estimation of Naproxen sodium
and Esomeprazole magnesium trihydrate in Pharmaceutical tablet dosage form. The separation was achieved by
using a reversed-phase C18column(Thermo eletrole, ODS, 250mm × 4.6 mm i.d, 5μm) at ambient temperature with
mobile phase consisting of Phosphate buffer (pH adjust to 3.8using OPA): Acetonitrile : Methanol (30:50:20v/v).
The flow rate was 1.0 ml/min. Detection was carried out at a wavelength of 220 nm. Retention time of Naproxen
sodium and Esomeprazole magnesium trihydrate were found tobe2.417 and 3.903min respectively. The proposed
method was validated for selectivity, precision, linearity and accuracy. The assay method was found to be linear
from 75-175µg/ml and 3-7µg/ml for Naproxen sodium and Esomeprazole magnesium trihydrate respectively. All
validation parameters were within the acceptable range. The developed method was successfully applied to estimate
the amount of Naproxen sodium and Esomeprazole magnesium trihydrate in Pharmaceutical tablet dosage form.
International Journal Of Pharmacy
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