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Title Formulation, Characterization, and Optimization of Snakehead Fish (Ophiocephalus Striatus) Powder Nanoemulgel
Authors Robert Tungadi, Prisca Wicita

Snakehead fish powder was formulated into nanoemulgel utilizing the best comparison of surfactant, co-surfactant and oil. The aims of this study was to determine physical stability and characterization of snakehead fish powder nanoemulgel by spontaneous emulsification method. PEG 400 was added into Tween 80 and olive oil then water, containing 0.1% of snakehead fish powder, was added drop by drop until becoming a transparent solution by sonication. Nanoemulsion was added with 1.5% HPMC gel and mixed until nanoemulgel form. It was characterized by UV-Vis Spectrophotometry and DLS. The results of this research showed that snakehead fish nanoemulgel produced clear, stable, and transparent formula having the transmittance value of 98.825%. The characterization results described nanoemulgel had the average of particle size, PDI, and zeta potential were around 2.9 nm, 0.589 and -60.72 mV respectively. This means that nanoemulgel was stable having a uniform particle size, pH 5, and the viscosity value of 210 cP. The results of the evaluation of stability test showed a good level of stability with the viscosity and pH by one way ANOVA analysis which did not change significantly.